Quantitative Analysis of Tissue Damage Evolution in Porcine Liver With Interrupted Mechanical Testing Under Tension, Compression, and Shear.

Joseph Chen; Bryn Brazile; Raj Prabhu; Sourav S. Patnaik; Robbin Bertucci; Hongjoo Rhee; M. F. Horstemeyer; Yi Hong; Lakiesha N. Williams; Jun Liao
J Biomech Eng. 2018 Jul 1;140(7). .

https://doi.org/10.1115/1.4039825

13 citations · 2 en 2025–2026

Les titres, résumés et légendes des publications sont présentés en anglais.

In this study, the damage evolution of liver tissue was quantified at the microstructural level under tensile, compression, and shear loading conditions using an interrupted mechanical testing method. To capture the internal microstructural changes in response to global deformation, the tissue samples were loaded to different strain levels and chemically fixed to permanently preserve the deformed tissue geometry. Tissue microstructural alterations were analyzed to quantify the accumulated damages, with damage-related parameters such as number density, area fraction, mean area, and mean nearest neighbor distance (NND). All three loading states showed a unique pattern of damage evolution, in which the damages were found to increase in number and size, but decrease in NND as strain level increased. To validate the observed damage features as true tissue microstructural damages, more samples were loaded to the above-mentioned strain levels and then unloaded back to their reference state, followed by fixation. The most major damage-relevant features at higher strain levels remained after the release of the external loading, indicating the occurrence of permanent inelastic deformation. This study provides a foundation for future structure-based constitutive material modeling that can capture and predict the stress-state dependent damage evolution in liver tissue.

Utilisation des équipements

Le Mach-1 Mechanical Tester a servi aux essais interrompus de traction, de compression et de cisaillement du parenchyme hépatique, avec les échantillons immergés dans du PBS dans une cuve sur mesure. Le chargement jusqu’à des déformations choisies était associé à une fixation chimique préservant le tissu déformé pour l’analyse microstructurale ultérieure. Des plaques de cisaillement sur mesure permettaient le mouvement relatif et le maintien des échantillons pendant la fixation.