Roger Y. Tam; Stephanie A. Fisher; Alexander E. G. Baker; Molly S. Shoichet
Chemistry of Materials 28 (2016), 3762–3770. Published 27 April 2016. Original publisher PDF deposited by the author laboratory at University of Toronto; title, authors, DOI, complete original abstract and Experimental Section visually checked. PDF p.2 explicitly documents uniaxial unconfined compression of HA-furan/PEG cryogels. Cells and other material assays are not assigned to Mach-1; no donor species is inferred from hyaluronan.
https://doi.org/10.1021/acs.chemmater.6b00627
51 citations · 2 en 2025–2026
Les titres, résumés et légendes des publications sont présentés en anglais.
Biomimetic scaffolds are desirable for 3D cell culture, yet optically transparent, macroporous, polysaccharide hydrogels have been limited. By incorporating soluble mono- and disaccharide additi ves during cryogelation, we synthesized biomimetic scaffolds and gained insight into the mechanism of their formation. The mono/disaccharide additives affect the size of ice crystal porogens and interact with polysaccharide polymers, altering cryogel pore size and mechanical properties. Importantly, gel transparency is maintained following removal of additives, enabling their use as biomimetic extracellular matrices. We demonstrate both optical transparency with 3D spatial control of immobilized bioactive growth factors using multiphoton patterning and cellular response to immobilized ligands.
Utilisation des équipements
Un Mach-1 mesurait le module de Young de cryogels HA–furane/PEG gonflés, en compression uniaxiale non confinée entre plateaux plats imperméables : précharge de 0,01 N, vitesse de 10 µm/s jusqu’à 20 % de déformation, à 37 °C. Au moins cinq gels étaient testés par formulation.
Matériaux (1)
Hydrogel
Configurations de test (1)
