Preparation and Characterization of Thermoresponsive Polymer Scaffolds Based on Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) for Cell Culture

Abstract

In the realm of scaffold-free cell therapies, there is a questto develop organotypic three-dimensional (3D) tissue surrogates in vitro, capitalizing on the inherent ability of cells to create tissues with an efficiency and sophistication that still remains unmatched by human-made devices. In this study, we explored the properties of scaffolds obtained by the electrospinning of a thermosensitive copolymer, poly(N-isopropylacrylamide-co-N-tert-butylacrylamide) (P(NIPAM-co-NtBA)), intended for use in such therapies. Two copolymers with molecular weights of 123 and 137 kDa and a content of N-tert-butylacrylamide of ca. 15 mol% were utilized to generate 3D scaffolds via electrospinning. We examined the morphology, solution viscosity, porosity, and thickness of the spun matrices as well as the mechanical properties and hydrophobic–hydrophilic characteristics of the scaffolds. Particular attention was paid to studying the influence of the thermosensitive polymer’s molecular weight and dispersity on the resultant scaffolds’ properties and the role of electroforming parameters on the morphology and mechanical characteristics of the scaffolds. The cytotoxicity of the copolymers and interaction of cells with the scaffolds were also studied. Our findings provide significant insight into approaches to optimizing scaffolds for specific cell cultures, thereby offering new opportunities for scaffold-free cell therapies.

The mechanical properties were analyzed with a Mach-1 v500csst micromechanical testing system (Biomomentum Inc., Laval, QC, Canada). The tensile strength, elongation at break and elasticity modulus (Young’s modulus) were measured upon the uniaxial tension of at least three dog bone-shaped samples (12 × 5 mm, transverse section, the thickness was taken from the SEM analysis) and were reported as mean ± standard deviation values.

Keywords:

Sacrificial fibers; electrospinning; fibrous materials; electrospun scaffolds; thermoresponsive polymers.