Osteochondral Allograft Transplantation: Tissue Storage and Surgical Factors Using an Ovine Model

An effective surgical treatment for large cartilage lesions is Osteochondral Allograft Transplantation (OCAT). The method and duration of cadaveric donor tissue storage prior to transplantation can reduce chondrocyte viability, which is directly correlated to negative clinical outcomes. Active research aims to increase chondrocyte viability at extended durations of storage. Effects of long-term storage were investigated by quantifying changes in ovine cartilage explants after storage in a proprietary, DMEM-based media known as MOPS. OCAT surgical techniques can also negatively impact cartilage quality. This thesis investigated the relationship between increased graft press-fit and insertion forces and resulting cell viability, using both human and ovine tissues. Finally, 3D-printed drill guides were developed for placement of osteochondral grafts in a pre-clinical ovine model. This thesis highlights interdependent factors that influence donor cartilage quality in OCAT, as well as identifying species differences that are important to consider when interpreting results from the pre-clinical ovine model.