Carroll Andrew; Anjum Fraz; Flynn Lauren; Amsden Brian
Oral# 442.7 on Saturday, May 21 from 16:30 to 18:30 in room 524 during World Biomaterials Congress, Montreal, May 2016
https://doi.org/10.3389/conf.fbioe.2016.01.02555
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Introduction: Chondroitin sulphate is an important component GAG found in articular cartilage and has a chondroinductive effect on adipose derived stem cells (ASCs). Methacrylate chondroitin sulphate (MCS) has therefore been chosen as a basis for a polymer vehicle for ASC delivery for chondral defect repair. However, MCS lacks cell binding site as well as the required mechanical strength and swells extensively following crosslinking. To overcome these limitations, MCS was conjugated with the peptide sequences GVOGEA, GLOGEN, and GGRGDS, and blended with acrylated triblock copolymers of poly(ethylene glcol)-block-poly(trimethylene carbonate) (A-PTMC-PEG-PTMC-A) then crosslinked using a thermal initiator. The resulting gels were assessed for their mechanical properties and ability to retain encapsulated ASC viability.
Methods: CS was reacted with glycidyl methacrylate in aqueous medium and subsequently functionalized with peptides via EDC coupling. Triblock copolymers were obtained via melt polymerization of PEG diol (20 kDa, 3.4 kDa) and TMC (5:1 mol:mol TMC:PEG); triblocks prepared with 20kDa PEG are referred to as 20k, while those with 3.4kDa PEG 3.4k. The block copolymers were acrylated with acryloyl chloride and characterized by 1H-NMR spectroscopy. Gels were formed from prepolymer solutions using the APS/TEMED initiator system (10 mM) by heating blended prepolymer solutions from 20 to 37C. Gels containing the 3.4k or 20k triblocks were made using a 1:1 (w/w) blend of triblock and MCS for a total polymer content of 20 wt%. Gel mechanical properties were measured on a MACH-1 micromechanical system and bovine ASC viability following encapsulation was assessed via LIVE/DEAD staining, confocal imaging, and image analysis using Image J.
Results: The MCS had a methacrylation degree of 11%, while the triblocks had acrylation degrees of > 95%. The equilibrium water content of all hydrogels was greater than 90 wt%. In uniaxial compression, ultimate stress, strain and tou
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Le Mach-1 mesurait les propriétés mécaniques d’hydrogels développés pour l’administration de cellules souches dérivées du tissu adipeux.
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